④ Numbering

Write a number in Indian ink on the side of the cut-out tissue opposite to the cut surface (i.e., thinly sliced surface).

Method

To remove moisture, gently press gauze onto the tissue area where the number will be written. After dipping the brush in ink, remove the excess ink with gauze or another suitable material. Write the number on the tissue taking care to avoid the sulcus.
After writing the number, gently press gauze onto the tissue to remove the excess ink.
Gently place the tissue in 70% ethanol.
Place a small tissue slice into a commercially available cassette and write the tissue number on the cassette.
Gently place this tissue in 70% ethanol.