④ Numbering

Write a number in Indian ink on the side of the cut-out tissue opposite to the cut surface (i.e., thinly sliced surface).


  • To remove moisture, gently press gauze onto the tissue area where the number will be written.
  • After dipping the brush in ink, remove the excess ink with gauze or another suitable material.
  • Write the number on the tissue taking care to avoid the sulcus.
  • After writing the number, gently press gauze onto the tissue to remove the excess ink.
  • Gently place the tissue in 70% ethanol.
  • Place a small tissue slice into a commercially available cassette and write the tissue number on the cassette.
  • Gently place this tissue in 70% ethanol.