⑪ Deparaffinization & hydration

These procedures are performed prior to staining.
Deparaffinization is to remove the paraffin penetrated into the tissue.
After deparaffinization, xylene is removed with 100% ethanol.
Then, slides are hydrated in a series of graded alcohols until water is used.

xylene IDeparaffinization
xylene II
xylene III
100% ethanol IRemoval of xylene
100% ethanol II
100% ethanol III
95% ethanolHydration
70% ethanol
Tap water


Issues to consider with deparaffinization