1	Deparaffinization	Xylene	3 changes, 10 minuntes each
2	Removal of xylene	100% ethanol	3 changes, 5 minutes each
3	Hydration	95%, 70% ethanol	5 minutes each
4	Washing	Running tap water	2 minutes
5	Rinsing	Distilled water
6	Staining	Azocarmine G solution (preheated at 50℃)	1 hour at 50℃ and 1 hour at RT (Filter before use)
7	Washing	Running tap water
8	Differentiating	Aniline-alcohol solution	Differentiate until nuclei stand out sharply (This step may be omitted)
9	Rinsing	Acetic alcohol	1 to 2 minutes (This step is omitted when sept8 is omitted.)
10	Washing	Running tap water
11	Mordanting	5% phosphomolybdic acid solution	3 hours or overnight
12	Washing	Running tap water
13	Rinsing	Distilled water
14	Staining	Aniline blue-orange G mixture	Approximately 15 to 30 minutes. After staining, wipe slides with filter paper to drain off the solution.
15	Differentiating	100% ethanol	3 changes. Agitate the slides during this step.
16	Microscopic check		Differentiate until blue and red color can be distinguished.
17	Clearing	Xylene	3 changes, 10 minuntes each
18	Coverslipping

Phosphotungstic acid (substituted by phosphomolybdic acid)	5g
distilled water	100ml

⑫ Staining

Azan staining