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① Fixation
② Washing in tap water
③ Dissecting Brains
④ Numbering
⑤ Dehydration
⑥ Clearing
⑦ Paraffin impregnation
⑧ Paraffin embedding
⑨ Mounting on a wooden block
⑩ Sectioning
Small blocks
Large paraffin blocks
⑪ Deparaffinization & hydration
⑫ Staining
HE staining
Nissl staining
LFB staining
KB staining
Woelcke staining
Bodian staining
Holmes staining
Holmes staining
(modification)
GB staining
Holzer staining
Marchi staining
Berlin blue staining
Kossa staining
Azan staining
IHC staining
Enzymatic method
IF method
Nuclear staining
⑬ Dehydration, clearing and coverslipping
③ Dissecting Brains
Dissect the brain tissue to the size of a paraffin block.
Method
Perform the dissecting procedure on a workbench equipped with a down-flow push-pull ventilation system to vent formalin fumes.
Equipment for dissecting
Photography stand for imaging the dissecting tissue.
Set up photocatalyst environmental purification equipment to deal with the formalin.